Keith Nehrke : Curriculum Vitae

Keith Nehrke
Assistant Professor of Medicine
Nephrology Unit
University of Rochester
Rochester, NY
office: (585) 275-7020
lab: (585) 273-4835
Email:Keith_Nehrke@urmc.rochester.edu

Education:
Degree	Discipline		Year	Institute

B.S. (honors)	Biochemistry		1989	SUNY Binghamton
Ph.D	Biochemistry		1994	University of Rochester

Postdoctoral Training:
Dates	Institute		Department	Field

1994 - 1998	University of Rochester		Center for Oral Biology	Glycobiology

Honors and Awards:

1994 - 1997	NIH Oral, Cell and Molecular Biology Postdoctoral Training Grant
1991 - 1994	NIH Mechanisms of Molecular Pathogenesis Pre-Doctoral Training Grant
1989 - 1991	Dean’s Fellowship, University of Rochester
1985 - 1989	Presidential Fellowship, SUNY Binghamton

Refereed Papers:		Generate a list of PubMed citations containing Nehrke, K [author].

1. Phizicky, E.M., Consaul, S.A., Nehrke, K.W., and Abelson, J. (1992)
Yeast tRNA Ligase Mutants are Nonviable and Accumulate tRNA Splicing Intermediates. J Biol Chem. 267, 4577-4582.
2. Nehrke, K., Seifried, S.E., and Platt, T. (1992)
Overproduced Rho Factor from p39AS has Lysine Replacing Glutamic Acid at Residue 155 in the Linker Region Between its RNA and ATP Binding Domains. Nucleic Acids Res. 20, 6107.
3. Nehrke, K., Zalatan, F., and Platt, T. (1993)
NusG Alters Rho-Dependent Termination of Transcription In Vitro Independent of Kinetic Coupling. Gene Exp. 3, 119-133.
4. Nehrke, K. and Platt, T. (1994)
Evidence for the Formation of a Quaternary Termination Complex: Rho, NusG, RNA Polymerase and the Nascent Transcript. J Mol Biol. 243, 830-839
5. Russnak, R., Nehrke, K., and Platt, T. (1995)
REF2 Encodes a Novel RNA-Binding Protein Directly Involved in Yeast mRNA 3' End Formation. Mol Cell Biol. 15, 1689-1697
6. Nehrke, K., Hagen, F.H., and Tabak, L.A. (1996)
Charge Distribution of Flanking Amino Acids Influences O-Glycan Aquisition in Vivo. J Biol Chem. 271, 7061-7065
7. Nehrke, K. and Tabak, L.A. (1997)
Biosynthesis of a Low Molecular Mass Rat Submandibular Gland Mucin in COS7 Cells. Biochem J. 323, 497-502
8. Nehrke, K. Ten-Hagen, K.G., Hagen, F.K.,and Tabak, L.A. (1998)
Charge Distribution of Flanking Amino Acids Inhibits O-Glycosylation of Several Single Site Acceptors In Vivo. Glycobiology 7,1053-1060
9. Nehrke, K., Hagen, F.K., and Tabak, L.A. (1998)
Isoform-specific O-glycosylation by Murine UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3, in vivo. Glycobiology 8, 367-371
10. Hagen, F.K. and Nehrke, K. (1998)
cDNA Cloning and Expression of UDP-N-acetyl-D-galactosamine:Polypeptide N-Acetylgalactosaminyltransferase sequence homologs in Caenorhabditis elegans J Biol Chem. 273, 8268-8277
11. Nehrke, K., Begenisich, T., Pilato, J., and Melvin, J.E. (2000)
C. elegans ClC-type Chloride Channels: Novel Variants and Functional Expression. Am J Physiol Cell Physiol., 279, C2052-C2066.
12. Park, K., Evans, R.L., Watson, G., Nehrke, K., Dennet, M.R., Richardson, L., Bell, S., Schultheis, P.J., Hand, A., Shull, G.E., and Melvin, J.E. (2001)
Defective Fluid Secretion and NaCl Absorption by Parotid Glands in Mice Lacking Na-H Exchange. J Biol Chem., 276, 27042-27050.
13. Melvin, J.E., Nguyen, H-V., Nehrke, K., Schreiner, C.M., Ten Hagen, K., and Scott, W. (2001)
Targeted Disruption of the Nhe1 Gene Fails to Inhibit b1-Adrenergic Receptor-Induced Parotid Gland Hypertrophy. Am J Physiol Gastrointest Liver Physiol., 280, G694-G700.
14. Yan, W., Nehrke, K., Choi, J. and Barber, D. (2001)
The Nck-interacting kinase NIK phosphorylates the Na-H exchanger NHE1 and regulates NHE1 activation by platelet-derived growth factor. J Biol Chem., 276, 31349-31356
15. Attaphitaya, S., Nehrke, K., and Melvin, J.E. (2001)
Acute Inhibition of Brain-Specific Na+/H+ Exchanger Isoform 5 by Protein Kinase A, Protein Kinase C and Cell Shrinkage. Am J Physiol Cell Physiol., 281, C1146-C1157
16. Elble, R., Gi, J., Nehrke, K., DiBiasio, J., Kingsley, P., Kotlikoff, M.I., and Pauli, B.U. (2002)
Molecular and functional characterization of a murine calcium-activated chloride channel expressed in smooth muscle. J Biol Chem., 277, 18586-18591
17. Nehrke, K., Arreola, J., Nguyen, H-V., Pilato, J., Richardson, L., Okunade, G., Shull, G.E., Baggs, R. and Melvin, J.E. (2002)
Saliva Secretion and Cell Volume Regulation by Parotid Gland Acinar Cells from Mice Lacking Expression of the Clcn2 Cl- Channel Gene. J Biol Chem. 277:23604-23611
18. Nehrke, K. and Melvin, J.E. (2002)
The NHX family of Na-H exchangers in Caenorhabditis elegans. J Biol Chem. 277:29036-29044
19. Arreola, J., Begenisich, T., Nehrke, K., Nguyen, H-V., Park, K., Richardson, L., Yang, B., Lamb, F., Shutte, B.C., and Melvin, J.E. (2002)
Secretion and Cell Volume Regulation by Salivary Acinar Cells from Mice Lacking Expression of the Clcn3 Cl- Channel Gene. J Physiol. 545:207-216
20. Dickerson, L.W., Schutte, B.C., Yang, B., Bonthius, D.J., Barna, T.J., Bailey, M.C., Nehrke, K., Williamson, R.A. and Lamb, F.S. (2002)
Altered GABAergic function accompanies hippocampal degeneration in mice lacking ClC-3 voltage-gated chloride channels. Brain Research 958:227-250.
21. Nehrke, K., Quinne, C.C., and Begenisich, T. (2003)
Molecular identification of the Ca2+-activated K+ channels in parotid acinar cells. Am J Physiol Cell Physiol. 284:C535-C546.
22. Joiner, W.J., Basavappa, S., Vidyasagar, S., Nehrke, K., Krishnan, S., Binder, H.J., Boulpaep, E.L., and Rajendran, V.M. (2003)
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon. Am J Physiol Gastrointest Liver Physiol. 285:G185-G196
23. Hoffman J.F., Joiner W., Nehrke K., Potapova O., Foye K., and Wickrema, A. (2003)
The hSK4 (KCNN4) isoform is the Ca2+-activated K+ channel (Gardos channel) in human red blood cells. Proc Natl Acad Sci U S A. 100:7366-7371
24. Nehrke, K. (2003)
A reduction in intestinal cell pHi due to loss of the C. elegans Na+/H+ exchanger NHX-2 increases lifespan. J Biol Chem 278:44657-44666
25. Chen, Y., Zhao, Y.H., Kalaslavadi, T.B., Hamati, E., Nehrke, K., Le, A.D., Ann, D.K.,and Wu, R. (2004)
Genome-wide search and identification of a novel gel-forming mucin MUC19/Muc19 in glandular tissues. Am J Respir Cell Mol Biol 30:155-165
26. Denton, J., Nehrke, K., Rutledge, E., Morrison, R. and Strange, K. (2004)
Alternative splicing of N- and C-termini of a C. elegans ClC channel alters gating and sensitivity to external Cl- and H+. J Physiol 555: 97-114.
27. Begenisich, T., Nakamoto, T., Ovitt, C., Nehrke, K., Brugnara, C., Alper, S.L., Melvin, J.E. (2004)
Physiological roles of the intermediate conductance, Ca2+-activated K channel, Kcnn4. J Biol Chem. [Epub ahead of print].
top
Invited Reviews:

1. Nehrke, K., Hagen, F.H., and Tabak, L.A. (1997)
Experimental Approaches to Studying O-Glycosylation In Vivo. In: Techniques in Glycobiology, Ed. R. Townsend and A. Hotchkiss. Marcel Dekker. New York, NY.
2. Hagen, F., Layden, M., Nehrke, K., Gentile, K., Berbach, K., Tsao, C.C., and Forsythe, M. (2001)
Mucin Type O-Glycosylation in C. elegans is Initiated by a Family of Glycosyltransferases. Trends in Glycoscience and Glycotechnology, 13 (73): 463-479
3. Melvin, J.E., Arreola, J., Nehrke, K., and Begenisich, T. (2002)
Ca2+-Activated Cl- Currents in Salivary and Lacrimal Glands. In: Current Topics in Membranes, vol. 53. Ed. C.M. Fuller. Academic Press. San Diego, CA.
top
National Conference Presentations (last three years):

Nehrke, K., Quinne, C., Melvin, J.E., and Begenisich, T. (2002, April)
Molecular identification of the Ca2+-activated K+ channels in parotid acinar cells.Poster presented at FASEB meeting, New Orleans, LA
Rutledge, E., Nehrke, K. and Strange, K. (2002, April)
Serine/threonine phosphorylation signaling pathways regulate the C. elegans* ClC anion channel CLH-3.* Poster presented at FASEB meeting, New Orleans, LA
Denton, J., Nehrke, K. and Strange, K. (2003, April)
Biophysical analysis of two C. elegans CLH-3 variants reveals a role of intracellular amino- and carboxyl-termini in channel gating and extracellular pH sensitivity. Poster presented at FASEB meeting, New Orlean, LA
Denton, J., Nehrke, K. and Strange, K. (2003, April)
Regulation of C. elegans* CLH-3b activation kinetics by an Ste20-related kinase.* Poster presented at FASEB meeting, New Orleans, LA
Nehrke, K. (2003, May)
The C. elegans* intestinal Na+-H+ exchanger NHX-2 influences nutrient uptake and aging.* Poster presented at AGA national meeting, Orlando, FL
Nehrke, K., Sherman, T., and Melvin, J.E. (2003, July)
A reduction in intestinal cell pHi due to loss of the C. elegans Na+/H+ exchanger NHX-2 increases lifespan. Lecture presented at the biennial International C. elegans meeting, Los Angeles, CA
Nehrke, K and Sherman, T. (2004, April)
Acid-base transport in C. elegans. Poster presented at FASEB meeting, Washington, DC
Denton, J., Nehrke, K., Rutledge, E., Morrison, R., and Strange, K (2004, April)
Alternative splicing of N- and C-termini of a C. elegans* ClC channel alters gating and sensitivity to external Cl- and H+.* Poster presented at FASEB meeting, Washington, DC
Denton, J., Nehrke, K., Morrison, R., and Strange, K (2004, April)
Molecular identification of a kinase that interacts with and regulates cell volume-dependent activity of a ClC anion channel. Poster presented at FASEB meeting, Washington, DC
top
Research Projects ongoing over the last three years:

USPHS R03 DE14119 "Molecular Basis of K+ Current During Secretion"
Principal Investigator: K. Nehrke
Dates of project: 04/01/01-03/31/03
USPHS R21 DK062763 “Na+/H+ Exchange in the Nematode Intestine”
Principal Investigator: K. Nehrke
Dates of project: 4/1/03-3/31/05
USPHS P30 AG018254 “Nathan Shock Center for Excellence in Aging Research”
Principal Investigator: H.J. Federoff
Pilot Project-“Intestinal Cellular pH as a Regulator of Longevity in C. elegans”
Dates of project: 7/1/04-6/30/05
USPHS R01 HL080810 “pH Regulation of Fat Storage in the Nematode Intestine”
Principal Investigator: K. Nehrke
Dates of project: 10/1/04-9/30/08
top
Professional Experience

July, 1989 to June, 1994
Graduate research associate to Dr. Terry Platt, Department of Biochemistry, University of Rochester. Research: RNA processing in E. coli and S. cerevisiae. Relevant techniques: yeast genetics, yeast two-hybrid analysis, classical enzyme kinetics, recombinant protein production, cell fractionation and protein purification, transcriptional regulation and RNA processing, molecular biology
July, 1994 to December, 1997
Postdoctoral research associate to Dr. Lawrence A. Tabak, Departmental Chair, Departments of Dental Research and Biochemistry; Director, Center for Oral Biology, University of Rochester. Research: Molecular mechanisms of mucin biosynthesis and glycosylation. Relevant techniques: cDNA library construction, molecular and in silico gene identification/screening, expressional cloning and purification of mammalian fusion proteins from E. coli, S. cerevisiae, P. pastoris and tissue culture cells, yeast two-hybrid analysis, oligosaccharide analysis, protein microsequencing, immunoassays, molecular biology.
January, 1998 to September, 2002
Assistant Research Professor in the laboratory of Dr. James E. Melvin, Professor and Director, Center for Oral Biology and Professor, Department of Pharmacology and Physiology, University of Rochester. Research: Ion Channel Function in Secretory Oral Epithelia. Relevant techniques: C. elegans genetics, reverse genetics and biochemistry, mouse targeted gene ablations, confocal microscopy, immunohistochemistry, cellular physiology and real time fluorescent imaging, electrophysiology, molecular biology.
October, 2002, to present
Assistant Professor of Medicine, Nephrology Unit, University of Rochester. Research: *i) Acid-Base Transport in C. elegans* and ii) Molecular Mechanisms of Calcium Homeostasis.**
top